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13% of all electrons transferred through the ETC leaks to generate ROS, particularly through complex I

Macrophage viability was determined by release of LDH into the culture medium as measured by the CyQuant TM Lactate Dehydrogenase assay kit (#C20300, Invitrogen, Eugene, OR) following the manufacturers protocol

[DOI] [PMC free article] [PubMed] [Google Scholar] 60.Demontis D., Walters G.B., Athanasiadis G., Walters R., Therrien K., Nielsen T.T., Farajzadeh L., Voloudakis G., Bendl J., Zeng B., et al