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Counts per gene were calculated using custom scripts acting in a HTSeqcount compatible mode with the following parameters: --format=bam --minaqual=10 --stranded=reverse mode=union (51, 52)

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Liquid chromatographymass spectrometry (LC-MS) has become an essential technique in this space, offering the sensitivity and specificity required to support the full lifecycle of GLP-1-based drug development, from early discovery through clinical validation

Other non-entero-pancreatic hormone-based pharmacotherapies such as bimagrumab and growth differentiation factor 15 (GDF-15) are also under investigation

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