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Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark

This dosage form not only retains the core biological activities of IGF-1 LR3, such as promoting cell proliferation and inhibiting apoptosis, but also reduces its damage to the external environment during storage and administration through the protective effect of the capsule shell, and improves the convenience of administration