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Characterising how specific amino acid changes in variant receptors alter binding affinity, Gs coupling efficiency, cAMP signalling, and MITF activation in response to MC1R agonist stimulation

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Immunohistochemistry The expression of matrix metalloprotein -9 (MMP-9) in the lung section was identified by immunohistochemistry staining with the primary antibody of MMP-9 (1:1,000 dilution, Abcam), according to the manufacturers instructions

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