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found that Ras Selective Lethal 3 can induce ferroptosis in primary melanocytes, as reflected by suppressed cell growth, mitochondrial dysfunction, increased ROS production and iron ion accumulation [76]

The reaction was stopped by adding 3x SDS sample buffer and boiling at 95 C for 10 min before the samples were separated by SDS-PAGE and chain cleavage was analyzed by immunoblotting using a primary anti-SUMO2 antibody (Thermo, cat no