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Intranasal administration of mouse [D-Leu-4]OB3, a synthetic peptide amide with leptin-like activity, enhances total uptake and bioavailability in Swiss Webster mice when compared to intraperitoneal, subcutaneous, and intramuscular delivery systems

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[1] Symptoms of B12 deficiency develop gradually and may include: Persistent fatigue and weakness Neurological symptoms such as paraesthesia (pins and needles), particularly in hands and feet Cognitive changes including memory problems or confusion Glossitis (sore, red tongue) and mouth ulcers Mood disturbances including depression or irritability Balance problems and difficulty walking Neurological complications can become irreversible if left untreated , making prompt diagnosis and treatment essential

Some patients report temporary fatigue, headache, or mild GI discomfort in the first 24 to 48 hours following an injection, particularly with their first dose