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Intracerebroventricular Administration Intracerebroventricular drug administration was performed as previously described ( (a) GLP-1R siRNA-1 Sense: GGCAUUGUCAAGUAUCUC UACGAGG Antisense: CCUCGUAGAGAUACUUGACAAUGCC (b) GLP-1R siRNA-2 Sense: GUUUCUGCCCUGCAUGG GUACUACT Antisense: AGUAGUACCCAUGCAGGGCAGAAAC (c) GLP-1R siRNA-3 Sense: AUAUCUGUGGAUGGUGUU GAAGATA Antisense: UAUCUUCAACACCAUCCCAGAUAT (d) Scrambled siRNA Sense: CGUUAAUCGCGUAUAAUA CGCGUAT Antisense: AUACGCGUAUUAUACGCGAU UAACGAC Dorsomorphin dihydrochloride (Santa Cruz Biotechnology Inc., TX, United States), a selective AMPK inhibitor, also known as compound C, was prepared at 10 mmol/L in 20% DMSO of PBS and injected (10 l) 30 min before SAH induction in SAH + Ex-4 + Dorsomorphin groups

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Injecting into the same spot repeatedly can lead to local tissue changes and increased injection site reactions

Zinc and copper are cofactors for collagen cross-linking enzymes