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The pbgst-ko 2A and 2B plasmids were generated using the pL0034 plasmid backbone (MRA-849, BEI Resources), which contains the positive-negative selectable marker cassette human dihydrofolate reductase/yeast cytosine deaminase and uridyl phosphoribosyl transferase ( hdhfr/yfcu ) under the control of the constitutive eukaryotic elongation factor 1A promoter