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Glatter: In practice, many patients labeled non-responders to GLP-1 therapy are experiencing incomplete dosing, early discontinuation because of gastrointestinal side effects, insufficient treatment duration, or competing metabolic drivers such as severe insulin resistance, sleep disruption, sarcopenia, or medication-associated weight gain

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RNA was precipitated by incubating the samples with 500 L of ice-cold isopropyl alcohol (Roth) and 100 L cold sodium acetate (3 M, pH 5.5) for 1 h at 20 C